Enhanced Metabolite Profiling: Hard and Soft Ionization High-Resolution Time-of-Flight Mass Spectrometry

This application note demonstrates the value of both electron impact ionization (EI) and chemical ionization—high-resolution time-of-flight mass spectrometry (HR-CI) in identifying analytes in complex metabolomic samples. The complementary use of EI and CI enhances the identification of metabolites through the preservation of molecular ions and improved spectral quality. The study showcases the application of these ionization techniques in analyzing a mouse liver extract, highlighting the benefits of HR-CI in metabolite profiling.